Native Reversed-Phase Liquid Chromatography: A Technique for LCMS of Intact Antibody–Drug Conjugates

Reversed-phase high-performance liquid chromatography.

EVALUATION OF THE USE OF ANIONIC/ NONIONIC MIXED MICELLES IN REVERSED PHASE LIQUID CHROMATOGRAPHY OF CHLOROPHENOLS

The use of aqueous mixed micellar system consisting of sodium dodecyl sulfate (SDS) and polyoxyethylene (23) dodecanol (Brij-35) as the mobile phase in reversed-phase liquid chromatography was studied. A group of chlorophenols was used as the test mixture. Adding organic modifier to the system showed that the use of low concentrations of organic additives improves efficiency in SDS/Brij-35 mixe...

Analysis of intact human follicle-stimulating hormone preparations by reversed-phase high-performance liquid chromatography.

A reversed-phase high-performance liquid chromatography (RP-HPLC) method for the qualitative and quantitative analysis of intact human follicle-stimulating hormone (hFSH) was established and validated for accuracy, precision and sensitivity. Human FSH is a dimeric glycoprotein hormone widely used as a diagnostic analyte and as a therapeutic product in reproductive medicine. The technique develo...

Separation of Somatropin and Its Degradation Products by High-Performance Liquid Chromatography Using a Reversed-Phase Polymeric Column

The accurate prediction of protein stability is one of the most challenging goals in protein formulation and delivery. In this study, a gradient RP-HPLC method is described for the separation of human growth hormone (hGH) variants as deamidated and oxidized forms. The methodology employed a polymeric poly (styrene-co-divinylbenzene) column and a 1mL/min flow rate of a linear gradient of 0.1% v/...

Urine by a Reversed- phase High-perfbrmance Liquid Chromatography

er than the normal amount. Especially, it is interesting that nicotinic acid reduces serum cholesterol and triglycerides.i) To study the unknown mechanism, the establishment of a practical method to measure nicotinic acid and its direct metabolite, nicotinuric acid, is important. In l978, Hengen et al.2] measured nicotinic acid and nicotinuric acid in plasma and urine with reversed-phase high-p...